OBJECTIVES
To achieve our overall objective of creating a pig GEM and tissue-specific GEMs for muscle and fat cells, we will:
Develop a GEM of Sus scrofa using the publicly available genome sequence and metabolic pathway databases. Further, constrain the GEM to create tissue-specific models based on transcriptomic data collected from primary pig cell lines.
Collect experimental data on biomass composition and growth rates in commercially available serum-free medium (from Van Heron Labs) to be used to develop the objective function in the flux balance analysis model (FBA). Validate/Test model predictions by directly measuring intracellular fluxes and performing spent media analysis of cells grown in serum-free medium.
Identify, engage with and replicate objectives 1-2 for a cultured meat company.
PROJECT PARTNERS
Industry Application and Benefits
Help to develop cost-effective media formulations
Current status
Ongoing (extended the research to other species)
Funding Support
